Table 2.

Fatty acid composition of total phospholipids, triacylglycerols and free fatty acid profile in L. donovani promastigote and amastigote

	Phospholipids		Triacylglycerols		Free fatty acids
	Pro	Ama	Pro	Ama	Pro	Ama
14:0	0.6 ± 0.3	3.0 ± 0.5**	2.6 ± 0.8	4.0 ± 1.5	1.9 ± 0.7	4.0 ± 1.5
16:0	23.5 ± 3.2	26.5 ± 4.4	3.9 ± 0.9	10.5 ± 1.0**	6.9 ± 0.6	23.3 ± 0.9**
18:0	10.0 ± 1.7	9.7 ± 1.3	10.6 ± 1.2	10.0 ± 2.2	13.4 ± 1.9	13.0 ± 2.5
Total saturate	34.1	39.2	17.1	24.5	22.2	40.3
16:1n-9	0.5 ± 0.3	0.9 ± 0.3	1.5 ± 0.8	3.4 ± 0.7*	0.5 ± 0.2	1.2 ± 0.5
16:1n-7	0.7 ± 0.3	8.0 ± 0.8***	0.5 ± 0.2	4.1 ± 0.8**	1.3 ± 0.5	7.0 ± 2.4*
18:1n-9	35.9 ± 2.1	20.1 ± 0.2***	29.6 ± 3.4	22.1 ± 3.6	20.2 ± 3.7	19.3 ± 0.6
18:1n-7	3.3 ± 1.1	18.3 ± 2.3***	1.6 ± 0.2	13.8 ± 4.0*	2.6 ± 1.0	20.5 ± 4.3**
Total monoenoic	40.4	47.3	33.2	43.4	24.6	48.0
18:2n-6	9.5 ± 1.2	3.4 ± 1.0*	8.6 ± 2.5	6.4 ± 1.0	13.4 ± 1.7	3.7 ± 1.4*
18:3n-6	10.6 ± 3.0	0.6 ± 0.3**	31.8 ± 1.5	15.3 ± 2.4***	31.3 ± 3.8	1.3 ± 1.0***
20:3n-6	3.1 ± 1.4	1.5 ± 0.5	3.5 ± 0.9	3.5 ± 0.1	4.6 ± 0.2	1.1 ± 0.5*
20:4n-6	0.7 ± 0.2	3.5 ± 1.0**	0.3 ± 0.1	2.1 ± 0.8*	0.5 ± 0.1	2.3 ± 0.8*
Total n-6	23.9	9.3	44.2	27.3	49.5	8.4
18:3n-3	nd	nd	nd	nd	nd	nd
20:5n-3	nd	0.7 ± 0.3*	nd	nd	nd	nd
22:5n-3	1.1 ± 0.9	1.6 ± 0.4	1.9 ± 0.3	1.9 ± 0.1	1.4 ± 0.9	1.7 ± 0.2
22:6n-3	0.5 ± 0.1	1.7 ± 0.6	3.6 ± 0.8	2.9 ± 0.3	2.0 ± 0.8	1.6 ± 0.1
Total n-3	1.6	4.2	5.5	4.8	3.4	3.3

Fatty acid composition was determined by GC analysis as described in materials and methods. Data, expressed as mole percent, are means of 3 independent experiments ± SD

* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 significant differences between promastigote and amastigote by t test