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. 2017 Mar 24;163(3):461–474. doi: 10.1007/s10549-017-4202-z

Fig. 4.

Fig. 4

EP300 regulates anchorage independence. Anchorage independence was determined by mammosphere formation in low attachment plates (ac) and the formation of clones in soft agar (de) after 2 and 4 weeks, respectively. Representative pictures of at least three independent experiments are shown. Numerical data indicate the anchorage independence efficiency after counting tumour spheres larger than 50 µm in diameter and is represented as the average ± SD of three independent experiments. All statistical comparisons (*P < 0.05) versus control cells