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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Nature. 2017 Apr 26;545(7653):229–233. doi: 10.1038/nature22312

Figure 4. A substantial fraction of hPSCs in published studies harbor TP53 mutations.

Figure 4

a-e, Published RNAseq data show that 7/117 (6%) unique hPSC lines harbor P53 mutations. f, Combined DNAseq and RNAseq analysis reveals 12/252 (5%) distinct cell lines affected by 15 TP53 mutations (Supplementary Table 3). g,h,i, P53 mutant WA01 was seen in three (g), WA09 acquired four distinct TP53 mutations in three groups (h), and WIBR3 lost all normal copies of TP53 after gene editing (i). j,k, TP53 mutant cells could be differentiated (j,k), and expanded relative to WT cells (k). Error bars depict SEM. l,m, Model of TP53 mutation enrichment during hPSC culture (l) or during clonal bottlenecks (m).