Figure 8.
Assessment of H2S in vivo using MitoA. A, MitoA in the heart during storage ex vivo. MitoA (50 nmol) was administered to mice via tail vein injection. The heart was removed after ∼12 min of warm ischemia. The hearts were then either snap frozen or stored for 240 min on ice and then snap frozen. Samples were analyzed by LC-MS/MS for MitoA and MitoN, and the MitoN/MitoA ratio was calculated. Results are mean ± S.E., n = 3. *, p < 0.05 by Student's two-tailed t test. B, protocol for the LAD artery occlusion experiments. C, mice were injected with 50 nmol of MitoA prior to ischemia of the left ventricle induced by occlusion of the LAD artery. At the end of the protocol the hearts were separated into right (normoxic) and left (normoxic or ischemic) ventricles, snap frozen, and analyzed for MitoA and MitoN content by LC-MS/MS, enabling the MitoN/MitoA ratio to be calculated. Data are mean ± S.E., n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, by two-way analysis of variance followed by post hoc Bonferroni test.