Figure 5.

The differential impact of Irs1 and Irs2 on the cellular response to microtubule disruption. A—G, PyMT cells were treated with DMSO or the indicated drugs for 48 h. The cells were stained with propidium iodide and analyzed by flow cytometry. Shown are the percentages of cells in the sub-G₁ peak (A and B) or cell cycle stages (C–G). The data shown represent the mean ± S.E. of representative experiments performed three times or twice (Taxol;Irs2 cells) independently. 2fl/fl, PyMT:Irs2^fl/fl cells; 2−/−, PyMT;Irs2^−/− cells; 2−/−:IRS2, PyMT:Irs2^−/−:IRS2 cells; 1−/−, PyMT;Irs1^−/− cells; 1−/−:IRS1, PyMT:Irs1^−/−:IRS1 cells. *, p ≤ 0.05 relative to Irs^fl/fl; **, p ≤ 0.001 relative to Irs^fl/fl.