Figure 7.

Involvement of the apoptotic effector BIM in the response to microtubule disruption. A and B, MDA-MB-231 cells were treated with DMSO or the indicated drugs for 48 h. Aliquots of cell extracts containing equivalent amounts of total protein were immunoblotted with antibodies specific for cleaved caspase 3 (A) or BIM (B) and tubulin. The data shown in the bottom panels represent the mean ± S.E. of three independent experiments. *, p ≤ 0.05 relative to DMSO; **, p ≤ 0.01 relative to DMSO; #, p ≤ 0.05 relative to shGFP-Nocodazole; ##, p ≤ 0.01 relative to shGFP-Nocodazole. Noc, nocodazole.