Figure 3.

VAMP8^−/− acini are fully protected from supramaximal CCK-induced intracellular trypsin accumulation and cell damage. A, total cellular trypsin activity in acinar lysates after enterokinase cleavage of trypsinogen was normalized to total DNA in WT and VAMP8^−/− acini. B and C, WT or VAMP8^−/− acini were left untreated (Basal) or stimulated with Max CCK-8 (10 pm in WT, 30 pm in VAMP8^−/−) or Supramax CCK-8 (10 nm in WT, 30 nm in VAMP8^−/−) for 30 min. Intracellular trypsin activity was measured and normalized to total DNA in panel B or to total cellular trypsin in panel C. D, WT or VAMP8^−/− acini were treated as above for 3 h, and lactate dehydrogenase was released to the medium was calculated as a percentage of total cellular levels. Note that VAMP8^−/− acini are fully protected from Supramax CCK-8 induced damage (data are the mean and S.E. *, p < 0.05). All experiments were generated from at least three separate acinar preparations, performed in duplicate.