Figure 7.

Enhanced endosomal trafficking prevents zymogen activation. WT mouse acini were cultured on collagen-coated plates in the presence of 10% FBS, 0.5 mm isobutylmethylxanthine, and 10⁶ pfu/ml adenovirus encoding GFP control, HA-D52, or canine Rab5 for 24 h. A, D52 and Rab5 levels as determined by immunoblot. B, amylase release after basal and supramaximal (100 nm) CCK-8 (30 min) stimulation. C, intracellular trypsin activity after basal and supramaximal CCK-8 stimulation (data are the mean and S.E. *, p < 0.05). All experiments were generated from at least three separate acinar preparations, performed in duplicate.