Figure 3.

Biochemical properties of TaRecJ2. A–C, TaRecJ2 (WT) and its mutant TaRecJ2 D34A/D36A (mt) were incubated at 50 °C with 50 nm 5′-FITC-labeled 30-nt ssDNA (A30), 30 bp of dsDNA (A30 + A30RC), 30 bp of dsDNA with a 15-nt 5′-overhang (A30temp5 + A30), 20 bp of dsDNA with a 25-nt 3′-overhang (A30temp3 + A30–11-30), or 20 bp of dsDNA/RNA and 10-nt 3′-RNA overhang (rA30 + A30RC-11–30). The reaction products were separated by native 20% PAGE (A) and denaturing 15% PAGE (B and C). Asterisks indicate the positions of fluorescent labeling. D, quantification of the degraded products shown in B. The decreased amount of substrate DNA was quantified and plotted at each time. The averages in three independent experiments with the S.E. are shown.