Figure 5.

HIF-1α transcriptional activity is not required for activation of Notch1 in prolonged CdCl₂-exposed A549 cells. A, prolonged 20 μm CdCl₂-exposed A549 cells (Cd) were transfected with control siRNA, ARNT siRNA-1, or ARNT siRNA-2. Cell lysates were subjected to Western blotting using the indicated antibodies. B and C, prolonged 20 μm CdCl₂-exposed A549 cells (Cd) were transfected with control siRNA or ARNT siRNA-1. Total RNAs were subjected to quantitative RT-PCR to determine VEGF-A mRNA levels. Data were normalized to β-actin expression and reflect the mean ± S.D. (error bars) of three experiments. *, p < 0.05; **, p < 0.01, significant difference between the samples (B). Cell lysates were subjected to Western blotting using the indicated antibodies (C). Immunoblots shown are representative of at least three independent experiments.