Figure 6.

Notch1 regulates HIF-1α expression in prolonged CdCl₂-exposed A549 cells. A, B, and C, prolonged 20 μm CdCl₂-exposed A549 cells were transfected with control siRNA, Notch1 siRNA-1, or Notch1 siRNA-2. Cell lysates were subjected to Western blotting using the indicated antibodies (A and C). Total RNAs were subjected to quantitative RT-PCR to determine HIF-1α mRNA levels. Data were normalized to β-actin expression and reflect the mean ± S.D. (error bars) of three experiments (B). D, prolonged 20 μm CdCl₂-exposed A549 cells were treated with 0.1% DMSO or 200 nm PPP for 16 h. Cell lysates were subjected to Western blotting using the indicated antibodies. Immunoblots shown are representative of at least three independent experiments. P-, phospho-.