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Ser-269 phosphorylation enhanced apical AQP2 retention in the mpkCCD cells. A, the experimental protocol. B and C, confocal immunofluorescence and immuno-EM micrographs of AQP2 in mpkCCD cells in response to the vasopressin V2 receptor-specific analog dDAVP. The apical plasma membrane was labeled with biotin. The cell nuclei were stained with DAPI. pS269 indicates Ser-269 phosphorylated AQP2 (arrowheads in C).
