Figure 4.

PRIP-deficient bone marrow cell cultures with M-CSF and RANKL. A, bone marrow cells from the femurs of both genotypes were cultured in 96-well plates with M-CSF for 3 days and then with M-CSF and RANKL for 4 days. The cells were stained for TRAP. TRAP-positive cells (TRAP⁺) containing more than three nuclei were regarded as osteoclasts, and the number of the cells is shown in the graph. Images and results shown are for males only. B, bone marrow cells were cultured as described in A in low-cell-binding plates. Cells stained with anti-RANK-phycoerythrin (PE) and anti-CD115-Alexa 488 antibodies were analyzed using flow cytometry. The panels are typical examples of the results, and the graph summarizes the analysis. Similar results were obtained for samples from three mice of each genotype cultured in triplicate. *, p < 0.05; **, p < 0.01. Bar = 20 μm.