Figure 3. MiR-449 mediated the promotive effect of EA on the expression of NSC biomarker Nestin and neuron biomarker NeuN. A. miR-449a agomir treatment (20 μl 500 pmol, intraperitoneal injection, for 3 days after SCI) promoted miR-449a level; B-D. miR-449a agomir treatment significantly restored the elevated mRNA (B) and protein (C, D) levels of Nestin, NeuN and CGRP by EA; E. Representative immunohistochemical stain of Nestin, NeuN and CGRP in ventral horn of spinal cord; F-H. Quantification of immunohistochemical stain of Nestin (F), NeuN (G) and CGRP (H); I-L. Protein level of TNF-α, IL-1β, cleaved caspase 3, Bax and Bcl2 measured with Western blotting, and quantified based on densitometry analysis. *P < 0.05, **P < 0.01, compared with SCI+EA group.
