Table 6. SD/aSD binding of nonhypothetical genes in B. subtilis phage φ29 in E. coli and B. subtilis.
| Gene | E. coli | B. subtilis | ||
|---|---|---|---|---|
| DtoStarta | SD | DtoStartb | SD | |
| gp2 | 14 | AAGGA | 17 | AAAGGA |
| gp3 | 17 | AAGGAG | 20 | GAAAGGAG |
| gp4 | 18 | AGGAGGU | 21 | AGGAGGU |
| gp5 | 15 | AAGGA | 18 | AAAGGA |
| gp6 | 19 | UAGAAAG | ||
| gp7 | 16 | GAGGUGA | 18,19 | UAGAAAG,GAGGUGA |
| gp8 | 18 | GAGGU | 21,21 | AGAAA,GAGGU |
| gp8.5 | 20 | GGAGGUG | 23 | GGAGGUG |
| gp9 | 16,19 | UAAGG,AGGUG | 22 | AGGUG |
| gp10 | 15 | GAGGUGA | 18 | GAGGUGA |
| gp11 | 16 | GGUGA | 19 | GGUGA |
| gp12 | 15 | UAAGGAGG | 18 | AAGGAGG |
| gp13 | 17 | GAGGU | 20 | GAGGU |
| gp14 | 17 | AAGGAG | 20 | AAAGGAG |
| gp15 | 17 | UAAGGAGG | 20 | AAGGAGG |
| gp16 | 16 | GAGGUG | 19 | GAGGUG |
Gene gp6, which uses a species-specific SDBs, cannot form a well-positioned SD/aSD in E. coli to be translated efficiently.
a
The optimal DtoStart is within the range of 10–21 in E. coli.
b
3′AUCUUUCCUCCACUAG is used as 3′TAIL for B. subtilis, with the optimal DtoStart within the range of 15–25.