Figure 2.

Notch is required and sufficient for LEP fate. All spermathecae are at 26–28 hr APF and at least 10 spermathecae are examined and show the same phenotype. (A–F) Expression of Lz (A and B), Hnt (C and D), and Cut (E and F) in spermathecal head. (A, C, and E) Spermathecae from the control group; (B, D, and F) depict spermathecae from N-knockdown pupae using lz-Gal4 (lz > N^RNAi). Yellow lines demarcate the apical and basal layers; LEPs in apical layers are pointed with arrows, whereas SUPs in basal layers are pointed with arrowheads. (G–I) Expression of Lz (G), Hnt (H), and Cut (I) in spermathecae with overexpression of lz-Gal4 driving Su(H)^DN. (J–L) Expression of Lz (J), Hnt (K), and Cut (L) in spermathecae with 51B02-Gal4 driving overexpression of NICD. APF, after puparium formation; DN, dominant negative; Hnt, Hindsight; LEP, lumen epithelial precursors; Lz, Lozenge; N, Notch; NICD, Notch intracellular domain; RNAi, RNA interference; Su(H), Suppressor of Hairless; SUP, secretory unit precursors.