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. 2017 Mar 3;7(5):1417–1427. doi: 10.1534/g3.117.040212

Figure 6.

Figure 6

Notch signaling is required for pIIb fate. (A and B) Representative control clones induced at 20 hr and examined at 48 hr APF. A three-cell clone is shown in (A), and a four-cell clone is shown in (B) with clone composition labeled according to Hnt expression (red). (C and D) Quantification of clone distribution according to clone size (C) and composition (D). The Fisher’s exact test was used (**P < 0.01 and ***P < 0.001). (E) A representative N-knockdown clone induced at 20 hr APF shows four BC-like cells without Hnt expression (red) at 48 hr APF. (F–H) Representative N-knockdown clones induced at 14 hr and examined at 48 hr APF. (F) A four-cell clone with four BCs (same size). (G) A four-cell clone with four BCs (two small and two big). (H) A four-cell clone with four BCs detaching from the lumen. APF, after puparium formation; BC, basal cell; GFP, green fluorescent protein; Hnt, Hindsight; N, Notch; RNAi, RNA interference.