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. 2017 Mar 3;7(5):1417–1427. doi: 10.1534/g3.117.040212

Figure 7.

Figure 7

Dl, but not Ser, is required for LEP fate specification. (A and B) Ser knockdown with lz-Gal4 shows normal Lz (A) and Hnt (B) expression in spermatheca at 27 hr APF. (C) Adult spermathecae with Ser knockdown in 51B02-Gal4-expressing cells have normal secretory cells marked by Hnt expression. (D–I) Dl knockdown in lz-Gal4- (D–F) or 51B02-Gal4-expressing cells (G–I) leads to loss of Lz (D and G) and gain of Hnt (E and H) expression at the middle region of spermathecal head at 27 hr APF. Few secretory cells were formed in adult spermathecae (F–I). (J–K) Dl-knockdown clones induced at 28 (J) or 24 hr (K) and examined at 48 hr APF. These clones have normal cell composition. Hnt expression (red) is used to marked the SCs. APF, after puparium formation; Dl, Notch ligand δ; Hnt, Hindsight; LEP, lumen epithelial precursor; Lz, Lozenge; RNAi, RNA interference; SC, secretory cell; Ser, Serrate.