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Dynamic evolution of gene families. The gene family expansions and contractions were predicted by CAFÉ 3.0. The species tree required by CAFÉ 3.0 was constructed by 1:1:1 single-copy gene families using the Maximum Likelihood method in MEGA6 (Tamura et al. 2013). The RelTime-ML program implemented in the MEGA6 package was used to estimate divergence time among species; calibration time was obtained from the TimeTree database.
