Figure 6.

The locomotive response to caffeine that is modified by amino acid changes in UNC-68 is dependent upon the chemosensory neuron–specific genes che-3 and osm-3. The locomotion of strains transgenic for the wild-type unc-68 (UL4140), for the EHI-associated variant R163C (UL4155), and for the LOAM-associated variant K3452Q (UL4168) was recorded upon RNAi knockdown of che-3 or osm-3 or in precisely equivalent blank control RNAi experiments. Mean body bends per minute in the presence of increasing concentrations of caffeine are presented for 50 individuals at 0 and 7 d of adulthood, i.e., young and old adults respectively. The color coding from Figure 1 is retained with, broadly, red for EHI and purple for LOAM. Error bars are SEM. Equivalent results for other strains are presented in Figure S1.