. 2017 May 12;17:112. doi: 10.1186/s12866-017-1013-7

Table 1.

Strains and plasmids used in this study

Strain or plasmid	Characteristics^a	Reference
E. piscicida
EIB202	Wild type (CCTC No. M 208068) carrying pEIB202, Col^r, Cm^r, Str^r	[33]
EIB202-lacZ ⁺	EIB202, insert a lacZ in glmS, Col^r, Str^r	Lab collection
EIB202 ∆P	EIB202, pEIB202 cured, Col^r	[41]
∆esrB	EIB202, in-frame deletion of esrB, Col^r, Cm^r, Str^r	[20]
∆topA	EIB202, in-frame deletion of topA, Col^r, Cm^r, Str^r	This study
∆p007	EIB202, in-frame deletion of p007, Col^r, Cm^r, Str^r	This study
∆virB9	EIB202, in-frame deletion of virB9, Col^r, Cm^r, Str^r	This study
esrB ⁺	EIB202, ∆esrB, containing pAKgfp1::flag-esrB, Col^r, Carb^r	Lab collection
topA ⁺	EIB202, ∆topA, containing pAKgfp1::topA, Col^r, Carb^r	This study
p007 ⁺	EIB202, ∆p007, containing pAKgfp1::p007, Col^r, Carb^r	This study
virB9 ⁺	EIB202, ∆virB9, containing pAKgfp1::virB9, Col^r, Carb^r	This study
∆P::pNQ705K	EIB202 ∆P with pNQ705K inserted in downstream of glms, Col^r, Km^r	This study
E. coli
DH5α λpir	Δ (lacZYA-argF) U169 (Φ80 LacZ ΔM15), pir dependent R6K.	Lab collection
SM10 λpir	thi thr leu tonA lacY supE recA::RP4–2-Tc::Mu, pir dependent R6K, Km^r	Lab collection
Vibrio harveyi
BB170	Vibrio harveyi, reporter strain, Carb^r	Lab collection
Vibrio alginolyticus
∆T6SS	Vibrio alginolyticus, T6SS genes were deleted, Carb^r	Lab collection
Plasmid
pMPR	Mariner transposon plasmid, pir dependent R6K, Gm^r, Carb^r	Lab collection
pAKgfp1	pBBRMCS4 with gfpmut3a	Lab collection
pNQ705	Suicide plasmid, pir dependent R6K, Cm^r	[53]
pNQ705K	pNQ705 derivative with Km fragment inserted in SalI site, Cm^r, Km^r	This study
pNQ705K-glmS	pNQ705K derivative with glmS fragment inserted in XbaI site, Cm^r, Km^r	This study
pDM4	Suicide plasmid, pir dependent, R6K, sacBR, Cm^r	[54]
pDMK	pDM4 derivative with Km fragment inserted in SalI site, Km^r, Cm^r	[25]
pD43B	pDMK derivative with virB10 and gyrB fragment inserted in XbaI site, Km^r, Cm^r	This study
p34S-Km	Cloning vector, Km^r	[55]

^aCol^r, polymyxin B resistance; Cm^r, chloroamphenicol resistance; Km^r, kanamycin resistance; Carb^r, carbenicillin resistance; Gm^r, gentamycin resistance