Fig. 3.

PLOD2 mediates the HIF-1α-stimulated migration and invasion of cells. a The effect of hypoxia was mimicked by cobalt chloride treatment (150 and 200 μM) for 24–72 h. Western blot showing an increase in PLOD2 and concurrent HIF-1α induction, with a maximum effect on 72 h. b, d Hypoxic cells pre-treated with cobalt chloride (150 μM) were then treated with minoxidil (0.5 mM) to inhibit PLOD2 expression. A migration assay (b) and matrigel-coated invasion assay (d) were conducted to compare the migratory and invasive ability of control cells, hypoxic cells, and hypoxic and PLOD2-inhibited cells. Photos were captured under a ×200 objective lens. c, e Statistical analyses of migration (c) and invasion (d) based on the mean ± SD of triplicate independent experiments. p values were obtained using Student’s t test. p < 0.05 is indicated by “**”, and p < 0.001 is indicated by “***”. f Scratch assays were conducted to support the results shown in b. Photos were captured under a ×200 objective lens. g Statistical analyses of scratch assay data (f) were conducted as described above