Fig. 1.

Antivenomics analyses of crotalic antivenom manufactured by IVB. a About 300 μg of crotamine-positive venom separated by RP-HPLC using second-generation antivenomics protocols [17]. The elution conditions were: isocratic gradient with 5% of 0.1% TFA in 100% of acetonitrile (solvent B) for 5 min followed by a 5–25% B for 5 min, 25–45% B for 60 min and 45–70% B for 10 min, with a final isocratic step of 70% B for 5 min at a flow rate of 1 mL/min. b and c the RP-HPLC profiles of retained and non-retained venom toxins on anticrotalic (batch SAC085204b) affinity column, respectively. Protein families associated with HPLC peaks: 1 –crotamine, 2 – disintegrin, 3–7 – crotoxin, and 8 – fractions of low expression toxins including D49-PLA₂, serine protease (gyroxin), C-type lectin (convulxin) and PIII-metalloproteases