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Limit of detection for the S and C regions using the duplex real-time PCR assay
| HBV load(IU/ml) | S region | C region | Duplex | |||
|---|---|---|---|---|---|---|
| Positive results/total tests | Positive rate (%) | Positive results/total tests | Positive rate (%) | Positive results/total tests | Positive rate (%) | |
| 100 | 20/20 | 100 | 20/20 | 100 | 20/20 | 100 |
| 50 | 20/20 | 100 | 20/20 | 100 | 20/20 | 100 |
| 20 | 20/20 | 100 | 20/20 | 100 | 20/20 | 100 |
| 10 | 14/20 | 70 | 13/20 | 65 | 18/20 | 90 |
| 5 | 6/20 | 30 | 9/20 | 45 | 10/20 | 50 |
| 2.5 | 4/20 | 20 | 5/20 | 25 | 7/20 | 40 |
