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. 2017 May 12;18:254. doi: 10.1186/s12859-017-1656-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Spectral analysis and size distribution of microbubbles. a Pseudo-colored GP images from 10 spectral image stacks of microbubbles labelled with C-Laurdan. Microbubbles were auto-segmented and analyzed using the spherical object mode of the Spectral Imaging Toolbox. Scale bar 30 μm. Color bar legend gives GP values. b Size distribution (diameter) of the segmented microbubbles (n = 71). c Distribution of mean GP values for the segmented microbubbles (n = 71)