Fig. 2.

Somatic extract of M. marshalli abrogates the development of allergic airway inflammation. Mice received OVA/alum or PBS on days 0 and 7 and challenged using nebulization with 1% OVA or PBS for 30 min, three times over the course of experiment. In addition, some mice were treated with 20 μg of somatic extract during sensitization with OVA and then challenged with 1% OVA. Mice were sacrified at day 17, and lung sections were prepared and stained with periodic acid Schiff staining (PAS) and hematoxylin and eosin (H&E) for observation of goblet cells metaplasia and inflammatory cells infiltration, respectively. Abbreviations: O, OVA-administered mice group (arrows indicate severe infiltration of inflammatory cells goblet cells metaplasia); S, somatic + OVA administered mice (arrows indicate mild infiltration of inflammatory cells and goblet cells metaplasia); P, PBS received mice (no inflammatory cells infiltration and goblet cells metaplasia)