Fig. 3.
In vivo transmission of H129-ΔTK-tdT (a) Schematic genome diagrams of H129-ΔTK-tdT and the helper viruses. H129-ΔTK-tdT was generated by replacing the thymidine kinase gene (TK, UL23) of H129-BAC with CMV promoter controlled tdTomato (tdT) gene and the selection marker of zeocin resistance gene (ZeoR). The helper viruses of AAV-TK-GFP and AAV-DIO-TK-GFP express TK and GFP under the control of EF1α promoter either in the Cre-independent or -dependent manner, respectively. b–c Transmission of H129-ΔTK-tdT tracing system in vivo. AAV-TK-GFP and H129-ΔTK-tdT were sequentially injected into LGN (b) and MOB (c) with the dose following the table described in Materials and methods. Representative upstream or downstream brain regions were examined as shown in the simplified connectivity paths. The representative results from 3 mice per group are shown. LGN, lateral geniculate nucleus; V1 (L4, L6), layer 4, layer 6 of primary visual cortex, MOE, main olfactory epithelium; MOB, main olfactory bulb; Pir, piriform; ACo, anterior cortical amygdaloid nucleus