Fig. 2.

Expression studies for MAGEB4 at mRNA and protein levels. GADPH was used as internal control for RT-PCR; individual control without reverse transcriptase added to each sample and labeled as RT(−). a RT-PCR results of MAGEB4 and GADPH for different human tissues. b RT-PCR results of MAGEB4 and GADPH for human testicular samples with different histology. RT(+) (well nos. 1–9), RT(−) (well nos. 10–18). Sertoli cell only (samples 1–3), maturation arrest at different stages (samples 4–7), normal fertile (sample 8), prepubescent (sample 9). c Immunohistochemistry for MAGEB4. Isotype control (i). Human adult testicular tissue (ii). MAGEB4 is detected in differentiating germ cells and some Sertoli cells. Scale bars 50 μm. Germ cell (arrow). Sertoli cell (arrowhead)