Fig. 2.

Schematic drawing showing approaches of polar body (PB) transfer. a PB1T: oocytes are enucleated and the first PBs (PB1s) are biopsied. Then, the PB1s are interchanged and fused with the enucleated oocytes. The reconstituted oocytes are inseminated by ICSI. b PB2T: oocytes are inseminated by ICSI and the PB1s are removed simultaneously. After fertilization, the female pronuclei are removed, and then the PB2s are biopsied and fused with the enucleated zygotes