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. 2017 Apr 28;11:36–46. doi: 10.2174/1874091X01711010036

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© 2017 Amodio et al.

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. (2) — Ubiquitylation of Sec23a in Hek293T cells. (A) SDS-PAGE and Coomassie-staining of Huh7 (H) and Hek293T (K) cells transiently transfected with 3X-FLAG-Sec23a at 48 h of expression. Total extracts (Lysates), the FLAG-immunoprecipitated proteins and the unbound proteins are showed as steps of the 3xFLAG-Sec23a purification. (B) Hek293T cells were co-transfected with the pHA-Ubiquitin expression vector and the 3xFLAG-Sec23a vector. Cell lysates were immunoprecipitated and checked by Western Blot with the indicated antibodies as in (Fig. 1).