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. 2017 Apr 28;11:36–46. doi: 10.2174/1874091X01711010036

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© 2017 Amodio et al.

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. (5) — Sec23a ubiquitylation affects COPII vesicles formation. Huh7 cells seeded on glass coverslips were transiently transfected with the wilde type Sec23a-GFP or the mutated isoforms (C432A-Sec23a-GFP or C449A-Sec23a-GFP) and after 48 h of transfection processed for immunofluorescence with Sec16 specific antibody. The mean number of Sec23a-GFP positive spots (mean ± SD) and the percent of Sec23a-GFP pixels colabeling with Sec16a pixels (mean ± SD) are reported in the Sec23a-GFP panels and in the merged panels respectively.