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. 2013 Nov 27;6(1):3–14. doi: 10.1007/s12551-013-0125-z

Fig. 2.

Fig. 2

Phosphatidylinositol-4,5-bisphosphate binds to the lysine-rich cluster of the PKCα-C2 domain. a Binding isotherm obtained by using isothermal titration calorimetry (ITC) to measure the titration of 30 mM vesicles containing a mixture of phosphatidylcholine/phosphatidylinositol-4,5-bisphosphate (PIP 2) (molar ratio 95:5) into a cell containing 60 μM of the C2α-D246N/D248N mutant in the presence of 1 mM CaCl2. b Binding isotherm obtained by using ITC to measure the titration of 10 mM vesicles containing a mixture of phosphatidylcholine/ phosphatidylinositol-4,5-bisphosphate (molar ratio 95:5) into a cell containing 60 μM of the C2α-K209A/K211A mutant in the presence of 1 mM CaCl2