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The activity inhibition of Zn2+ on SPAK. Different concentrations of Zn2+ was incubated with SPAK for 2 h in buffer (20 mM Tris-acetic acid, pH 8.0) at 20°C, followed the relative activity measurement with corresponding Zn2+ concentrations (A) or without Zn2+ (B). The final concentration of SPAK was 0.06 μM. Data are presented as average values, n=3.
