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. 2017 Mar 3;7:46. doi: 10.1038/s41598-017-00163-2

Figure 4.

Figure 4

Effect of NF-κB blockade on ER stress and insulin signaling. Tunicamycin (Tun) was used to induce insulin resistance. HUVECs were cultured in the presence or absence of uncarboxylated osteocalcin with or without 1 μM PDTC (an NF-κB inhibitor) and 100 nM NF-κB-p65 siRNA. For insulin signaling, cells were stimulated with 10 nM of insulin for 10 min. The relative quantity of proteins was analyzed Quantity One software. (A) Protein expression of NF-κB-p65 in HUVECs. (B) Phosphorylation of PERK and IRS-1 in HUVECs. (C) Densitometric analyses of PERK and IRS-1 in HUVECs. A representative blot from three independent experiments is shown and the data expressed as mean ± SEM in each bar graph represent the average of three independent experiments. *P < 0.05 (Tun/ucOcn vs. Tun/Veh). # P < 0.05 (Tun/ucOcn/inhibitor vs. Tun/ucOcn). ΔP < 0.05 (p65 siRNA vs. control siRNA). IB, immunoblot; IP, immunoprecipitation.