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. 2017 Mar 7;7:97. doi: 10.1038/s41598-017-00162-3

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© The Author(s) 2017

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Histological examination using hemtoxylin-eosin-saffron staining. (A) Control sclerotic-equivalent zone (x200); (B). Necrotic zone (ON group) (x200); (C). Sclerotic zone (ON group) (x200); (D). Sclerotic zone with coexisting normal trabeculae, woven and chondroid bone (ON group) (x200); (E). pyknotic apoptotic osteocytes (ON group) (x400); (F). coexistence of pyknotic osteocytes others presenting with nuclei showing spread and faded chromatin (x400). ^&Normal trabecula, ^$woven bone, ^¥chondroid bone, ^#normal medullary space, ^§necrotic medullary space, *intact osteocyte, ^¶apoptotic osteocyte, ¤empty osteocyte lacuna. ON: osteonecrosis.