Table 1.

PCR primers used for koala retrovirus (KoRV) quantification in the blood of free-living koalas (Phascolarctos cinereus).

Target	Specificity	Primer Set	Product Sizea	Reference
env gene	Subgroup A	Universal F: 5′-TCCTGGGAACTGGAAAAGAC-3′ A_R: 5′-GGGTTCCCCAAGTGATCTG-3′	321	This study
env gene	Subgroup B	Universal F: 5′-TCCTGGGAACTGGAAAAGAC-3′ B_R: 5′-GGCGCAGACTGTTGAGATTC-3′	271	This study
pol gene	All KoRVs	F: 5′-TTGGAGGAGGAATACCGATTACAC-3′ R: 5′-GCCAGTCCCATACCTGCCTT-3′	111	Tarlinton et al. 21

^aExpected size predicted from alignment with the KoRV subgroup A and B representative genome sequences with Genbank accessions AF151794 and KC779547.