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. 2017 Mar 3;7:62. doi: 10.1038/s41598-017-00108-9

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of GSPs on mitochondrial membrane potential in irradiated HFL1 cells. (A) Flow cytometry analysis of JC-1 staining was used to assess mitochondrial membrane potential (MMP). HFL1 cells were pre-treated with GSP (10 μg/ml) or MitoQ (200 nmol/L) for 24 h before irradiation, and MMP was determined at 72 h after irradiation. One representative flow cytometry plot of the mitochondrial membrane potential is shown out of three replicates. The cells with enhanced red fluorescence had a higher membrane potential. The numbers in the corners represent the percentage of cells in the corresponding quadrants. (B) The percentage of cells with high MMP (%) is shown. Data are expressed as the mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 versus control group. ^##P < 0.01 versus model group.