Figure 8.

Effect of GSPs on the expression and enzyme activity of complex I in irradiated HFL1 cells. (A) HFL1 cells were pre-treated with GSPs (10 μg/ml), MitoQ (200 nmol/L) for 24 h before irradiation. The cells were immunostained with a complex I antibody at 72 h after irradiation. A representative confocal image from three replicates is shown. Scale bars: 20 µM. (B) Complex I enzyme activity was measured using spectrophotometry and expressed as ΔmOD/min/mg protein. The data were expressed as the mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 versus control group. ^#P < 0.05 and ^##P < 0.01 versus model group.