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. 2013 Oct 1;27(11):1934–1945. doi: 10.1210/me.2013-1099

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Copyright © 2013 by The Endocrine Society

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Figure 3. — MRAP2s and MC4R interactions. HEK cells were transfected with Flag-tagged MRAPs and/or Myc-tagged MC4R. Whole-cell lysates were prepared 24 hours after transfection and used for Western blot or incubated with anti-FLAG magnetic beads or anti-MYC agarose beads for coimmunoprecipitation. Both Flag-MRAP2a and Flag-MRAP2b, but not Flag-MRAP1, interact with Myc-MC4R as seen by immunoblotting (IB) with anti-Flag and anti-Myc, respectively after immunoprecipitation (IP) with anti-Myc. Asterisks indicate positive bands in crude lysates and immunoprecipitated samples whereas white arrowheads show unspecific bands.