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Immunofluorescence assays in live cells showing the expression of MRAPs (green) and/or zfMC4R (red). N-terminally Flag-tagged MRAPs and N-terminally Myc-tagged MC4R were transiently expressed in HEK-293 cells. Photomicrographs are taken with a ×60 objective and are from a single optical section obtained within an acquisition of z stacks (0.10 μm/slice). Arrows indicate regions of the plasma membrane where MRAPs and MC4R are potentially colocalized.
