Figure 8.

A, Effects of MRAP2a and MRAP2b on MC4R-induced galactosidase basal activity in HEK-293 cells stably expressing MC4R but transiently expressing galactosidase gene under the control of a constitutive promoter carrying several CRE sites (see Material and Methods for more details). A construct carrying luciferase gene under the control of a constitutive promoter was also transfected to standardize transfection levels. Experiments were performed using quadruplicate data points and repeated 4 independent times. Asterisks show significant differences after Student's t test (P < .05). B, Total and cell surface detection of Myc-zfMC4R using anti-Myc antibodies. Control corresponds to nontransfected HEK-293 cells. Cells were transiently transfected with MC4R or MC4R+MRAP2a and assayed for total and extracellular c-Myc detection by whole-cell ELISA. The results represent the mean ± SEM of 3 independent experiments, each performed in triplicate.