Table 2.
MC4R Was Expressed Alone or in Combination With One or Several MRAPs in HEK-293 Cells Expressing a Reporter Gene Under The Control of CREs
| MC4R | MC4R MRAP2a | MC4R MRAP2a MRAP1 | MC4R MRAP2a MRAP2b | MC4R MRAP2a MRAP2b MRAP1 | MC4R MRAP2b MRAP1 | MC2R | MC2R MRAP1 | MC2R MRAP2a MRAP1 | MC2R MRAP2a | |
|---|---|---|---|---|---|---|---|---|---|---|
| ACTH (1–24) | 8.6 × 10−7 | 9.59 × 10−9 a | 9.87 × 10−9 a | 4.97 × 10−9 a | 9.64 × 10−8 a | — | — | 1.37 × 10−9 | 1.05 × 10−9 | — |
| [±0.36 × 10−6] | [±6.56 × 10−9] | [±3.39 × 10−9] | [±0.97 × 10−9] | [±1.88 × 10−9] | [±0.21 × 10−9] | [±1.13 × 10−9] |
Data were treated as in Table 1. ED50 values from gene reporter activation for ACTH(1–24) on zebrafish MC4R or MC2R and MRAPs expressed in HEK 293 cells. Dashes indicates non-significant fitting.