Table 2.
Summary of Glutamate and Glycine EC50 for wild type and mutant GluN2A constructs.
| Mutation | Glutamate EC50 µM, 95%CI (n) | Glycine EC50 µM, 95%CI (n) |
|---|---|---|
| WT | 0.42, 0.40–0.47 (16) | 0.55, 0.50–0.61 (12) |
| P79R | 1.45, 1.35–1.57 (15)*** | 1.53, 1.37–1.70 (11)*** |
| C231Y | 1.89, 1.71–2.10 (17)*** | 4.13, 3.86–4.42 (13)*** |
| C436R | — | — |
| G483R | 11.30, 10.1–12.65 (12)*** | 3.50, 3.01–4.08 (12)*** |
| M705V | 0.89, 0.78–1.02 (12)*** | 1.31, 1.11–1.53 (11)*** |
| E714K | 0.41, 0.38–0.45 (12) | 0.78, 0.72–0.85 (13) |
| D731N | — | |
| I814T | 0.42, 0.38–0.44 (9) | 0.58, 0.50–0.67 (10) |
| D933N | 0.41, 0.38–0.44 (9) | 0.68, 0.62–0.75 (10) |
| N976S | 0.37, 0.32–0.43 (9) | 0.71, 0.60–0.83 (11) |
Data expressed as mean (95% confidence intervals). N = number of wells from the single cell calcium imaging assays (3–6 assays). When no values are given this is because there was no detectable response. ***P < 0.0001 Bonferroni corrected extra-sum-of-squares F-test of best-fit values of LogEC50.