Table 1.

15-mer Peptide candidates tested individually for CD8⁺ T cell cytokine release.

Pool #	Peptide #	Sequence	% IFNγ + CD8 + T cells	Anchor Compatible Class I H-2 allele
Pool 5	33	QTWLESREYTKHLIR	0.029
	34	ESREYTKHLIRVENW	0.000
	35	YTKHLIRVENWIFRN	0.140
	36	LIRVENWIFRNPGFA	0.007
	37	ENWIFRNPGFALAAA	0.022
	38	FRNPG F ALAAAAIAW	0.490	Kb
	39	GFALAAAAIAWLLGS	0.021
	40	AAAAIAWLLGSSTSQ	0.100
Pool 6	41	IAWLLGSSTSQKVIY	0.029
	42	LGSSTSQKVIYLVMI	0.000
	43	TSQKVIYLVMILLIA	0.037
	44	VIYLVMILLIAPAYS	0.053
	45	VMILLIAPAYSIRCI	0.016
	46	LIAPA Y SIRCIGVSN	0.320	Kb
	47	AYSIRCIGVS N RDFV	0.440	Db
	48	RCIGVS N RD F VEGMS	0.380	Db/Kb
Pool 7	49	VSNRDFVEGMSGGTW	0.170
	50	DFVEGMSGGTWVDVV	0.045
	51	GMSGGTWVDVVLEHG	0.027
	52	GTWVDVVLEHGGCVT	0.018
	53	DVVLEHGGCVTVMAQ	0.017
	54	EHGGCVTVMAQDKPT	0.027
	55	CVTV M AQDKPTVDIE	0.350	Db
	56	MAQDKPTVDIELVTT	0.000
Pool 11	81	NLEYRIMLSVHGSQH	0.046
	82	RIMLSVHGSQHSGMI	0.009
	83	SVHGSQHSGMIVNDT	0.009
	84	SQHSGMIVNDTGHET	0.000
	85	GMIVNDTGHETDENR	0.027
	86	NDTGHETDENRAKVE	0.009
	87	HETDE N RAKVEITPN	0.340	Db
	88	ENRAKVEITP N SPRA	0.350	Db

Peptides that induced IFNγ expression in >0.2% of CD8⁺ T cells were considered hits. Possible anchoring residues are underlined.