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. 2017 Mar 3;7:56. doi: 10.1038/s41598-017-00156-1

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effects of the three embryo developmental stages on embryogenic tissue induction. Cleavage polyembryony-, dominant-, and precotyledon-stage embryos were cultured in embryogenic tissue induction media CM1–CM11 for 6 weeks, after which, the rates of callus (represented by blue columns) and ESM (represented by red columns) induction from CM1–CM7 and the survival rate of embryos (represented by green columns) from CM8–CM11 were determined. Duncan's new multiple range test was used to detect differences at P < 0.05.