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. 2017 Mar 3;7:56. doi: 10.1038/s41598-017-00156-1

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© The Author(s) 2017

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Effects of proliferation media PM1 and PM2 on cultures proliferation. (a) Further development of embryogenic callus in proliferation medium PM1. (b) Cultures mainly induced by cleavage polyembryony embryos dedifferentiated into soft and easy browning callus in proliferation medium PM2. (c) Embryogenic suspensor mass structures of the embryogenic callus observed under a microscope; these structures consisted of clusters of small meristematic cells with dense cytoplasm forming emerging embryonal heads with attached elongated suspensor cells. (d) Cultures mainly induced by precotyledon embryos dedifferentiated into hard and difficult to proliferate callus in medium PM2. (a,b,d) bars 1 mm, (c) bar 100 μm.