Skip to main content
. 2017 Mar 3;7:56. doi: 10.1038/s41598-017-00156-1

Figure 6.

Figure 6

Effect of different plant growth regulators combination on somatic embryogenesis. (a) Callus induced by somatic embryo induction medium SEI1. (b) Swollen embryos in somatic embryo induction medium SEI2. (c) Cup-shaped or somewhat curved-shaped initial buds emerged from the surface of swollen embryos in medium SEI2. (d) Elongating adventitious buds in subsequent cultures. (e) White cap-shaped or columnar pro-embryos arose from a disaggregated watery callus in SEI1 medium. (f) Partly dedifferentiated callus. (g,h) Growing adventitious buds from a partly dedifferentiated callus in medium SEI2. (i) Completely dedifferentiated callus characterized by white translucent gullies and bulges attached to the surface. (j–l) White cap-shaped or columnar pro-embryos arose from a disaggregated watery callus in SEI2 medium. Hundreds of pro-embryos were observed during further proliferation and laurel-green cotyledons appeared from the embryonal head. Bars 5 mm.