Figure 9.

Effects of ABA and PEG on the potential for somatic embryogenesis. (a–e) Early somatic embryo developmental potential after 2 weeks’ cultivation of embryogenic calluses in maturation media supplemented with five concentrations of ABA (0, 25, 50, 75, and 100 μmol L⁻¹) respectively. (f–h) Early somatic embryo developmental potential in maturation media supplemented with three concentrations of PEG6000 (50, 100, and 150 g L⁻¹), respectively. (i) Early somatic embryo developmental potential in maturation media supplemented with 50 μmol L⁻¹ ABA and 100 and 150 g L⁻¹ PEG6000. (j–l) As maturation progressed, early somatic embryos (j) gradually differentiated into early cotyledonary embryos (k) and finally developed into mature embryos (l). (a–h) bars 5 mm, (i–l) bars 1 mm.