Table 1.
Embryogenic tissue induction media (CM1–CM11).
Growth regulators | Callus induction mediuma (mg L−1) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
CM1 | CM2 | CM3 | CM4 | CM5 | CM6 | CM7 | CM8 | CM9 | CM10 | CM11 | |
2,4-D | 1.0 | 1.0 | 2.0 | 2.0 | 0.5 | 1.5 | 1.5 | — | — | — | 0.2 |
BA | 0.2 | 0.5 | 0.2 | 0.5 | 0.2 | — | 0.5 | 1.0 | 1.5 | 2.0 | 1.5 |
KN | — | 0.3 | 0.3 | — | — | 0.3 | 0.3 | — | — | — | — |
NAA | — | — | — | — | — | — | — | 0.2 | 0.2 | 0.2 | |
TDZ | — | — | — | — | — | — | — | 0.004 | 0.004 | 0.004 | 0.004 |
aEach medium was composed of DCR basal medium and 30 g L−1 sucrose, 0.5 g L−1 casein hydrolysate, 0.4 g L−1 glutamine, 5 g L−1 phytagel, 1.0 g L−1 activated carbon, and plant growth regulators.