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. 2017 Mar 3;7:56. doi: 10.1038/s41598-017-00156-1

Table 1.

Embryogenic tissue induction media (CM1–CM11).

Growth regulators Callus induction mediuma (mg L−1)
CM1 CM2 CM3 CM4 CM5 CM6 CM7 CM8 CM9 CM10 CM11
2,4-D 1.0 1.0 2.0 2.0 0.5 1.5 1.5 0.2
BA 0.2 0.5 0.2 0.5 0.2 0.5 1.0 1.5 2.0 1.5
KN 0.3 0.3 0.3 0.3
NAA 0.2 0.2 0.2
TDZ 0.004 0.004 0.004 0.004

aEach medium was composed of DCR basal medium and 30 g L−1 sucrose, 0.5 g L−1 casein hydrolysate, 0.4 g L−1 glutamine, 5 g L−1 phytagel, 1.0 g L−1 activated carbon, and plant growth regulators.