Table 2.

Effect of plant growth regulators combination on somatic embryogenesis.

Somatic embryo induction mediuma	Plant growth regulators (mg L−1)					Response (%)
	2,4-D	NAA	6-BA	KN	TDZ	Buding	SE	Callus
SEI1	0.5	—	—	0.5	—	0	2.62 ± 0.21	97.38 ± 0.21
SEI2	—	0.2	1.5	—	0.004	40.04 ± 0.92	8.45 ± 0.66	51.51 ± 0.79
SEI3	1.5	—	—	0.2	—	0	0	100
SEI4	1.5	—	0.2	—	—	0	0	100
SEI5	1.5	—	0.2	0.2	0.004	0	0	100

^aEach somatic embryo induction medium was composed of DCR basal medium and 30 g L⁻¹ sucrose, 0.5 g L⁻¹ casein hydrolysate, 0.4 g L⁻¹ glutamine, 5 g L⁻¹ phytagel, 1.0 g L⁻¹ activated carbon, and plant growth regulators.